Human UBE1L(Ubiquitin Activating Enzyme E1 Like Protein) ELISA Kit

Human

UBA7, D8, UBA1B, UBE1L, UBE2, ubiquitin like modifier activating enzyme 7, Ubiquitin-activating enzyme 7, Ubiquitin-activating enzyme E1 homolog

P41226

Sandwich

100mIU/mL

0.94 mIU/mL

1.57-100mIU/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Immune molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UBE1L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to UBE1L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UBE1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UBE1L in the samples is then determined by comparing the OD of the samples to the standard curve.