Human GPER1(G Protein Coupled Estrogen Receptor 1) ELISA Kit

Human

G protein-coupled receptor 3,GPR3,GPER, CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, LERGU, LERGU2, LyGPR

Q99527

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Tissue homogenate, Cell lysate

Signal transduction;CD & Adhesion molecule;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPER1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GPER1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPER1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GPER1 in the samples is then determined by comparing the OD of the samples to the standard curve.