Human MAPKAPK3(Mitogen Activated Protein Kinase Activated Protein Kinase 3) ELISA Kit

Human

MAPKAPK3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3, mitogen-activated protein kinase-activated protein kinase 3, MDPT326

Q16644

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Immune molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAPKAPK3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MAPKAPK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAPKAPK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MAPKAPK3 in the samples is then determined by comparing the OD of the samples to the standard curve.