Human NP(Nandrolone Phenylpropionate) ELISA Kit

Human

NPP, Nandrolone phenpropionate, 19-Nortestosterone phenylpropionate, Nandrolone hydrocinnamate, 19-Nortestosterone 17β-phenylpropionate

Competitive

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NP in the samples is then determined by comparing the OD of the samples to the standard curve.