Human CYSLTR2(Cysteinyl Leukotriene Receptor 2) ELISA Kit

Human

CYSLT2, CYSLT2R, HG57, HPN321, KPG_11, hGPCR21, GPCR21, PSEC146,G-protein coupled receptor GPCR21,G-protein coupled receptor HG57,HPN321

Q9NS75

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Ophthalmology & Otorhinolaryngology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYSLTR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CYSLTR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYSLTR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CYSLTR2 in the samples is then determined by comparing the OD of the samples to the standard curve.