Human PIK3Cσ(Phosphoinositide-3-Kinase Catalytic Delta Polypeptide) ELISA Kit

Human

PIK3CD, APDS, IMD14, P11DELTA, PI3K, p11D, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta

O00329

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Enzyme & Kinase;Neuro science;Developmental science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PIK3Cσ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIK3Cσ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIK3Cσ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIK3Cσ in the samples is then determined by comparing the OD of the samples to the standard curve.