Human PIK3Cβ(Phosphoinositide-3-Kinase Catalytic Beta Polypeptide) ELISA Kit

Human

PIK3CB, P11BETA, PI3K, PI3KBETA, PIK3C1, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta,PI3-kinase subunit beta,PI3K-beta,PI3Kbeta,PtdIns-3-kinase subunit beta,Phosphatidylinositol 4,5-bisphosphate 3-kinase 11 kDa catalytic subunit beta,PtdIns-3-kinase subunit p11-beta,p11beta

P42338

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PIK3Cβ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIK3Cβ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIK3Cβ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIK3Cβ in the samples is then determined by comparing the OD of the samples to the standard curve.