Human γ-ECS(γ-Glutamyl Systeine Synthetase) ELISA Kit

Human

GCS heavy chain,Gamma-ECS,Gamma-glutamylcysteine synthetase,Glutamate--cysteine ligase catalytic subunit,GCLC,GCL, GCS, GLCL, GLCLC

P48506

Sandwich

15ng/mL

0.14 ng/mL

0.24-15ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Enzyme & Kinase;Neuro science;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to γ-ECS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to γ-ECS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain γ-ECS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of γ-ECS in the samples is then determined by comparing the OD of the samples to the standard curve.