Human MAPτ(Microtubule Associated Protein Tau/Tau Protein) ELISA Kit

Human

MAPT, DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, TAU

P10636

Sandwich

500pg/mL

4.69 pg/mL

7.82-500pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;Infection immunity;Human gene deletion;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAPτ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MAPτ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAPτ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MAPτ in the samples is then determined by comparing the OD of the samples to the standard curve.