Human CXCR1(CXC-Chemokine Receptor 1) ELISA Kit

Human

Interleukin 8 receptor, alpha,Chemokine C-X-C motif Receptor 1,CKR1 ,CD181, CDw128a, CMKAR1, CXCR1, IL8R1, IL8RBA, IL8-RA,

P25024

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Tissue homogenate, Cell lysate

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXCR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CXCR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CXCR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CXCR1 in the samples is then determined by comparing the OD of the samples to the standard curve.