Human CPP(Copeptin) ELISA Kit

Human

CT-proAVP, C-Terminal Provasopressin, Vasopressin-Neurophysin 2-Copeptin

P01185(126~164aa)

Competitive

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Infection immunity;Endocrinology;Bone metabolism;Rheumatology;

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CPP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CPP in the samples is then determined by comparing the OD of the samples to the standard curve.