Human CASP8(Caspase 8) ELISA Kit

Human

CASP8, ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5, caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, MORT1-associated ced-3 homolog

Q14790

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;CD & Adhesion molecule;Enzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CASP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CASP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CASP8 in the samples is then determined by comparing the OD of the samples to the standard curve.