Human ANP(Atrial Natriuretic Peptide) ELISA Kit

Human

NPPA, ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND, Atrial natriuretic peptide, natriuretic peptide A

P01160(124~151aa)

Competitive

500pg/mL

4.69 pg/mL

7.82-500pg/mL

1.5H

Plasma

Tumor immunity;

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ANP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ANP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ANP in the samples is then determined by comparing the OD of the samples to the standard curve.