Human ISG15(Interferon Stimulated Gene 15) ELISA Kit

Human

G1P2, IFI15, IP17, UCRP, Interferon-induced 17 kDa protein, ISG15 ubiquitin-like modifier

P05161

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Endocrinology;Neuro science;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ISG15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ISG15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ISG15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ISG15 in the samples is then determined by comparing the OD of the samples to the standard curve.