Human TNFSF14(Tumor Necrosis Factor Ligand Superfamily, Member 14) ELISA Kit

Human

CD258, HVEML, LIGHT, LTg, TR2

O43557

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Cardiovascular biology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFSF14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNFSF14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFSF14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNFSF14 in the samples is then determined by comparing the OD of the samples to the standard curve.