Human IL-1α(Interleukin 1 Alpha) ELISA Kit

Human

IL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha

P01583

Sandwich

250pg/mL

2.35 pg/mL

3.91-250pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Cardiovascular biology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL-1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL-1α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL-1α in the samples is then determined by comparing the OD of the samples to the standard curve.