Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit

Human

PK1, EGVEGF, PRK1, PROK1, Prokineticin 1, Black Mamba Toxin-Related Protein, Mambakine

P58294

Sandwich

500pg/mL

4.69 pg/mL

7.82-500pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.