通用型ELISA试剂盒

孕蛋白(PREG)酶联免疫吸附测定试剂盒

货号

FY-EU1221

样本类型

Serum, Plasma and Other biological fluids

检测时间

1.5H

价格(美元)

规格

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产品详情
英文名称:
PREG(Pregnenolone) ELISA Kit
种属:
Universal
别称:
Pregnenolone
检测方法:
Competitive
标准品:
1000 pg/mL
灵敏度:
6.12 pg/mL
检测范围:
15.63-1000 pg/mL
反应时间:
1.5H
样品类型:
Serum, Plasma and Other biological fluids
研究领域:
Reproductive science
测试原理:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PREG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PREG. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PREG in the samples is then determined by comparing the OD of the samples to the standard curve.
技术数据
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
1000 0.273 0.274 0.274
500 0.373 0.374 0.374
250 0.567 0.571 0.569
125 0.828 0.834 0.831
62.5 1.123 1.125 1.124
31.25 1.518 1.531 1.525
15.63 1.832 1.842 1.837
0 2.496 2.503 2.5
精密度
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
回收率
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
样本类型 Range(%) Average Recovery(%)
Serum (n=8) 90-107 98
EDTA plasma (n=8) 87-99 93
Cell culture media (n=8) 82-98 90
线性
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 93-102 85-100 88-102
Average (%) 101 100 93
1:4 Range (%) 90-99 85-100 86-93
Average (%) 92 93 88
1:8 Range (%) 95-106 84-98 91-104
Average (%) 96 87 104
1:16 Range (%) 87-101 95-101 87-98
Average (%) 94 99 89
检测流程图
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