维生素D(VD)酶联免疫吸附测定试剂盒

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维生素D(VD)酶联免疫吸附测定试剂盒

货号

FY-EU1134

样本类型

Serum, Plasma and Other biological fluids

检测时间

1.5H

价格(美元)

280 400

规格

48T 96T

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DataSheet

说明书 MSDS

MSDS

产品详情

英文名称:

VD(Vitamin D) ELISA Kit

种属:

Universal

别称:

VD; VD1; Lamisterol; Vitamin D; Vitamin D1; Lumisterol

检测方法:

Competitive

标准品:

400 ng/mL

灵敏度:

2.19 ng/mL

检测范围:

6.25-400 ng/mL

反应时间:

1.5H

样品类型:

Serum, Plasma and Other biological fluids

研究领域:

Metabonomics

测试原理:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VD protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VD. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VD in the samples is then determined by comparing the OD of the samples to the standard curve.

技术数据

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD
400	0.24	0.241	0.241
200	0.403	0.404	0.404
100	0.643	0.648	0.646
50	0.96	0.969	0.965
25	1.253	1.257	1.255
12.5	1.573	1.586	1.58
6.25	1.843	1.857	1.85
0	2.665	2.674	2.67

精密度

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

回收率

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

样本类型	Range(%)	Average Recovery(%)
Serum (n=8)	88-102	95
EDTA plasma (n=8)	82-97	89
Cell culture media (n=8)	90-105	97

线性

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-104	83-95	83-95
1:2	Average (%)	95	94	83
1:4	Range (%)	82-93	83-95	97-105
1:4	Average (%)	83	93	104
1:8	Range (%)	81-96	85-96	88-95
1:8	Average (%)	94	90	91
1:16	Range (%)	83-94	87-101	96-102
1:16	Average (%)	86	91	99

检测流程图