GnRH(GonadotropinReleasing Hormone) ELISA Kit

Universal

GNRH1, GRH, HH12, LHRH, LNRH, Luliberin

Competitive

1000 pg/mL

9.38 pg/mL

15.63-1000 pg/mL

1.5H

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids

Signal transduction;Enzyme & Kinase;Infection immunity;

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GnRH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.