EasyPro Universal 3-Nitrotyrosine(3-NT)ELISA Kit

Universal

3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine

Competitive

300ng/mL

1.35 ng/mL

4.69-300 ng/mL

45min

Serum, plasma and other biological fluids

Metabolic pathway;Infection immunity;Rheumatology;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 3-NT Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for 3-NT are added to the micro ELISA plate wells. 3-NT in samples (or standards) competes with a fixed amount of 3-NT on the solid phase supporter for sites on the HRP linked detection antibody specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.