EasyPro Universal Epinephrine(EPI)ELISA Kit

Universal

Adrenaline

Competitive

2000pg/mL

9.74 pg/mL

31.25-2000 pg/mL

45min

Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Metabolic pathway;Infection immunity;Neuro science;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with EPI Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for EPI are added to the micro ELISA plate wells. EPI in samples (or standards) competes with a fixed amount of EPI on the solid phase supporter for sites on the HRP linked detection antibody specific to EPI. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of EPI in the samples is then determined by comparing the OD of the samples to the standard curve.