EasyPro Universal Dopamine(DA)ELISA Kit

Universal

2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride

Competitive

1000pg/mL

4.71 pg/mL

15.63-1000 pg/mL

45min

Serum, plasma and other biological fluids

Signal transduction;Cardiovascular biology;Reproductive science;Neuro science;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DA Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for DA are added to the micro ELISA plate wells. DA in samples (or standards) competes with a fixed amount of DA on the solid phase supporter for sites on the HRP linked detection antibody specific to DA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of DA in the samples is then determined by comparing the OD of the samples to the standard curve.