EasyPro Universal GonadotropinReleasing Hormone(GnRH)ELISA Kit

Universal

GNRH1, GRH, HH12, LHRH, LNRH, Luliberin

Competitive

1000pg/mL

9.38 pg/mL

15.63-1000 pg/mL

45min

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Signal transduction;Enzyme & Kinase;Infection immunity;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GnRH Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for GnRH are added to the micro ELISA plate wells. GnRH in samples (or standards) competes with a fixed amount of GnRH on the solid phase supporter for sites on the HRP linked detection antibody specific to GnRH. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.