EasyPro Rat Atrial Natriuretic Peptide(ANP)ELISA Kit

Rat

NPPA, ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND, Atrial natriuretic peptide, natriuretic peptide A

Competitive

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Metabolic pathway;Cardiovascular biology;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ANP Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for ANP are added to the micro ELISA plate wells. ANP in samples (or standards) competes with a fixed amount of ANP on the solid phase supporter for sites on the HRP linked detection antibody specific to ANP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of ANP in the samples is then determined by comparing the OD of the samples to the standard curve.