EasyPro Rat Deoxypyridinoline(DPD)ELISA Kit

Rat

Pyrilinks-D, deoxyPYD

O89000

Competitive

200ng/mL

1.88 ng/mL

3.13-200ng/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Enzyme & Kinase;Metabolic pathway;Neuro science;Developmental science;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DPD Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for DPD are added to the micro ELISA plate wells. DPD in samples (or standards) competes with a fixed amount of DPD on the solid phase supporter for sites on the HRP linked detection antibody specific to DPD. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of DPD in the samples is then determined by comparing the OD of the samples to the standard curve.