EasyPro Rat Prostaglandin F2 Alpha(PGF2α)ELISA Kit

Rat

Amoglandin, Croniben, Cyclosin, Dinifertin, Enzaprost, Glandin, PGF2α, Panacelan, Prostamodin

Competitive

500pg/mL

4.69 pg/mL

7.82-500pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Enzyme & Kinase;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGF2α Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for PGF2α are added to the micro ELISA plate wells. PGF2α in samples (or standards) competes with a fixed amount of PGF2α on the solid phase supporter for sites on the HRP linked detection antibody specific to PGF2α. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.