快速检测系列ELISA试剂盒
一步法小鼠D-二聚体(D2D)酶联免疫吸附测定试剂盒
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货号
FY-EM2841S样本类型
Plasma and other biological fluids；Sample Volume=50μL检测时间
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DataSheet 
产品详情
英文名称:
EasyPro Mouse D-Dimer(D2D)ELISA Kit
种属:
Mouse
别称:
D2D
检测方法:
Competitive
标准品:
5000ng/mL
灵敏度:
46.88 ng/mL
检测范围:
78.13-5000 ng/mL
反应时间:
45min
样品类型:
Plasma and other biological fluids；Sample Volume=50μL
研究领域:
Enzyme & Kinase;Metabolic pathway;Neuro science;Developmental science;
测试原理:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with D2D Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for D2D are added to the micro ELISA plate wells. D2D in samples (or standards) competes with a fixed amount of D2D on the solid phase supporter for sites on the HRP linked detection antibody specific to D2D. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of D2D in the samples is then determined by comparing the OD of the samples to the standard curve.
技术数据
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
| Concentration | OD1 | OD2 | Average OD | Corrected |
|---|---|---|---|---|
| 5000 | 0.162 | 0.165 | 0.164 | |
| 2500 | 0.3 | 0.303 | 0.302 | |
| 1250 | 0.519 | 0.524 | 0.522 | |
| 625 | 0.85 | 0.855 | 0.853 | |
| 312.5 | 1.271 | 1.284 | 1.278 | |
| 156.25 | 1.728 | 1.732 | 1.73 | |
| 78.13 | 2.037 | 2.052 | 2.045 | |
| 0 | 2.517 | 2.528 | 2.523 |
精密度
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
| Item | Intra-assay Precision | Intra-assay Precision |
|---|---|---|
| Sample | 3 | 3 |
| Replicate | 9 | 18 |
| CV(%) | 5 | 8 |
回收率
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| 样本类型 | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 80-95 | 87 |
| EDTA plasma (n=8) | 86-99 | 92 |
| Cell culture media (n=8) | 80-95 | 87 |
线性
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 80-93 | 95-103 | 91-103 |
| Average (%) | 88 | 95 | 93 | |
| 1:4 | Range (%) | 89-97 | 95-103 | 85-94 |
| Average (%) | 92 | 97 | 91 | |
| 1:8 | Range (%) | 85-94 | 86-92 | 87-101 |
| Average (%) | 85 | 87 | 100 | |
| 1:16 | Range (%) | 87-101 | 89-97 | 86-97 |
| Average (%) | 90 | 96 | 97 |
检测流程图
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