EasyPro Mouse Adrenocorticotropic Hormone(ACTH)ELISA Kit

Mouse

Corticotropin, Adrenocorticotropin

Competitive

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Tumor immunity;Cardiovascular biology;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ACTH Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for ACTH are added to the micro ELISA plate wells. ACTH in samples (or standards) competes with a fixed amount of ACTH on the solid phase supporter for sites on the HRP linked detection antibody specific to ACTH. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.