EasyPro Mouse Brain Natriuretic Peptide(BNP)ELISA Kit

Mouse

NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B

P40753

Competitive

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Endocrinology;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for BNP are added to the micro ELISA plate wells. BNP in samples (or standards) competes with a fixed amount of BNP on the solid phase supporter for sites on the HRP linked detection antibody specific to BNP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of BNP in the samples is then determined by comparing the OD of the samples to the standard curve.