EasyPro Mouse Melatonin(MT)ELISA Kit

Mouse

N-Acetyl-5-Methoxytryptamine

Competitive

500pg/mL

4.69 pg/mL

7.82-500pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Endocrinology;Gastroenterology;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MT Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for MT are added to the micro ELISA plate wells. MT in samples (or standards) competes with a fixed amount of MT on the solid phase supporter for sites on the HRP linked detection antibody specific to MT. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of MT in the samples is then determined by comparing the OD of the samples to the standard curve.