EasyPro Human Beta-Endorphin(β-EP)ELISA Kit

Human

b-EP

P01189(237~267aa)

Competitive

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Metabolic pathway;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-EP Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for β-EP are added to the micro ELISA plate wells. β-EP in samples (or standards) competes with a fixed amount of β-EP on the solid phase supporter for sites on the HRP linked detection antibody specific to β-EP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of β-EP in the samples is then determined by comparing the OD of the samples to the standard curve.