EasyPro Human Somatostatin(SST)ELISA Kit

Human

GHIH, SRIF, SMST, GHRIH, GHRIF, Growth Hormone Releasing Inhibiting Hormone, Somatotropin Release-Inhibiting Factor, Somatostatin-14, Somatostatin-28

P61278

Competitive

500pg/mL

4.69 pg/mL

7.82-500pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Enzyme & Kinase;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SST Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for SST are added to the micro ELISA plate wells. SST in samples (or standards) competes with a fixed amount of SST on the solid phase supporter for sites on the HRP linked detection antibody specific to SST. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of SST in the samples is then determined by comparing the OD of the samples to the standard curve.