快速检测系列ELISA试剂盒

一步法人促肾上腺皮质激素释放激素(CRH)酶联免疫吸附测定试剂盒
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货号

FY-EH3022S

样本类型

Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL

检测时间

45min

价格(美元)

规格

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产品详情
英文名称:
EasyPro Human CRH(Corticotropin Releasing Hormone) ELISA Kit
种属:
Human
别称:
CRF, Corticoliberin
Uniport ID:
P06850
检测方法:
Competitive
标准品:
250pg/mL
灵敏度:
2.35 pg/mL
检测范围:
3.91-250pg/mL
反应时间:
45min
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL
研究领域:
Endocrinology;Neuro science;Hormone metabolism;
测试原理:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CRH Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for CRH are added to the micro ELISA plate wells. CRH in samples (or standards) competes with a fixed amount of CRH on the solid phase supporter for sites on the HRP linked detection antibody specific to CRH. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of CRH in the samples is then determined by comparing the OD of the samples to the standard curve.
技术数据
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
250 0.159 0.159 0.159
125 0.336 0.337 0.337
62.5 0.541 0.548 0.545
31.25 0.851 0.866 0.859
15.63 1.193 1.201 1.197
7.82 1.575 1.593 1.584
3.91 1.835 1.847 1.841
0 2.466 2.481 2.474
精密度
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
回收率
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
样本类型 Range(%) Average Recovery(%)
Serum (n=8) 80-94 87
EDTA plasma (n=8) 95-107 101
Cell culture media (n=8) 82-95 88
线性
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 93-106 88-98 95-103
Average (%) 105 92 102
1:4 Range (%) 87-99 87-103 80-97
Average (%) 98 93 92
1:8 Range (%) 85-93 89-97 93-102
Average (%) 92 89 99
1:16 Range (%) 80-96 85-94 87-103
Average (%) 89 87 99
检测流程图
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