一步法小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

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货号

FY-EM1060S

样本类型

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

检测时间

1.5H

价格(美元)

300 420

规格

48T 96T

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DataSheet

产品详情

英文名称:

EasyPro Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

种属:

Mouse

别称:

DIF, TNF-alpha, TNFA, TNFSF2,TNF,TNFa

Uniport ID:

P06804

检测方法:

Sandwich

标准品:

200pg/mL

灵敏度:

0.32 pg/mL

检测范围:

3.13-200pg/mL

反应时间:

1.5H

样品类型:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

研究领域:

Ovarian dysfunction

测试原理:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNF-α. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for TNF-α are added to the micro ELISA plate wells. TNF-α in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to TNF-α. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-α. You can calculate the concentration of TNF-α in the samples by comparing the OD of the samples to the standard curve.

技术数据

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
200	2.333	2.337	2.335	2.313
100	1.531	1.543	1.537	1.515
50	0.928	0.942	0.935	0.913
25	0.496	0.497	0.497	0.475
12.5	0.241	0.242	0.242	0.22
6.25	0.126	0.126	0.126	0.104
3.13	0.065	0.066	0.066	0.044
0	0.022	0.022	0.022	0

精密度

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

回收率

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

样本类型	Range(%)	Average Recovery(%)
Serum (n=8)	80-93	86
EDTA plasma (n=8)	80-95	87
Cell culture media (n=8)	95-107	101

线性

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-104	85-92	98-105
1:2	Average (%)	96	88	102
1:4	Range (%)	87-96	88-96	96-103
1:4	Average (%)	92	92	98
1:8	Range (%)	89-101	87-98	87-95
1:8	Average (%)	101	90	92
1:16	Range (%)	97-105	95-102	83-92
1:16	Average (%)	105	97	88

检测流程图