EasyPro Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Rat

HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor

O35800

Sandwich

500pg/mL

4.69 pg/mL

7.82-500pg/mL

1.5H

Tissue homogenate, Cell lysate；Sample Volume=50uL

Enzyme & Kinase;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HIF-1α. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for HIF-1α are added to the micro ELISA plate wells. HIF-1α in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to HIF-1α. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HIF-1α. You can calculate the concentration of HIF-1α in the samples by comparing the OD of the samples to the standard curve.