EasyPro Rat Interleukin 5(IL-5)ELISA Kit

Rat

IL5, EDF, TRF, E-CSF, ESGF

Q08125

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Neuro science;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-5. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for IL-5 are added to the micro ELISA plate wells. IL-5 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to IL-5. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-5. You can calculate the concentration of IL-5 in the samples by comparing the OD of the samples to the standard curve.