一步法小鼠高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒

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货号

FY-EM3059S

样本类型

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

检测时间

1.5H

价格(美元)

300 420

规格

48T 96T

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DataSheet

说明书 MSDS

MSDS

产品详情

英文名称:

EasyPro Mouse HMGB-1(High Mobility Group Protein B1) ELISA Kit

种属:

Mouse

别称:

HMGB1, HMG1, HMG3, SBP-1, Amphoterin

Uniport ID:

P63158

检测方法:

Sandwich

标准品:

1000pg/mL

灵敏度:

9.38 pg/mL

检测范围:

15.63-1000pg/mL

反应时间:

1.5H

样品类型:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

研究领域:

Cytokine;Infection immunity;

测试原理:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HMGB-1. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for HMGB-1 are added to the micro ELISA plate wells. HMGB-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to HMGB-1. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HMGB-1. You can calculate the concentration of HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

技术数据

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
1000	2.465	2.465	2.465	2.429
500	1.585	1.595	1.59	1.554
250	0.892	0.903	0.898	0.862
125	0.457	0.466	0.462	0.426
62.5	0.248	0.253	0.251	0.215
31.25	0.142	0.144	0.143	0.107
15.63	0.091	0.093	0.092	0.056
0	0.035	0.036	0.036	0

精密度

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

回收率

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

样本类型	Range(%)	Average Recovery(%)
Serum (n=8)	80-93	85
EDTA plasma (n=8)	80-95	86
Cell culture media (n=8)	85-97	91

线性

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	85-101	85-92	80-96
1:2	Average (%)	87	90	90
1:4	Range (%)	80-96	82-96	89-102
1:4	Average (%)	80	91	96
1:8	Range (%)	87-99	87-103	85-97
1:8	Average (%)	89	94	96
1:16	Range (%)	96-107	95-102	80-97
1:16	Average (%)	104	102	94

检测流程图