英文名称:
EasyPro Mouse MIγ/CXCL9(Monocyte Interferon Gamma Inducing Factor) ELISA Kit
别称:
Chemokine(C-X-C motif) ligand 9,CXCL9, Monokine induced by gamma interferon,MIG,CMK, Humig, SCYB9, crg-1
样品类型:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL
研究领域:
Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
测试原理:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MIγ/CXCL9. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for MIγ/CXCL9 are added to the micro ELISA plate wells. MIγ/CXCL9 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to MIγ/CXCL9. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MIγ/CXCL9. You can calculate the concentration of MIγ/CXCL9 in the samples by comparing the OD of the samples to the standard curve.