英文名称:
EasyPro Human SP-A1(Pulmonary surfactant-associated protein A1) ELISA Kit
别称:
MGC133365, PSAP, PSPA, SFTP1, SFTPA1
样品类型:
Serum, Tissue homogenate, Cell lysate, Cell culture supernatant, Alveolar lavage fluid(Serum, tissuehomogenates, celllysates, cellculturesupernates, lunglavagefluid);Sample Volume=50uL
测试原理:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SP-A1. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for SP-A1 are added to the micro ELISA plate wells. SP-A1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to SP-A1. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SP-A1. You can calculate the concentration of SP-A1 in the samples by comparing the OD of the samples to the standard curve.