一步法人 Hornerin蛋白(HRNR)

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快速检测系列ELISA试剂盒

货号

FY-EH3647S

样本类型

serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

检测时间

45min

价格(美元)

300 420

规格

48T 96T

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产品详情

英文名称:

EasyPro Human Hornerin (HRNR)ELISA Kit

种属:

Human

检测方法:

Sandwich

标准品:

5000pg/mL

灵敏度:

46.88 pg/mL

检测范围:

78-5,000pg/mL

反应时间:

45min

样品类型:

serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

测试原理:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with HRNR Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for HRNR are added to the micro ELISA plate wells. HRNR in samples (or standards) competes with a fixed amount of HRNR on the solid phase supporter for sites on the HRP linked detection antibody specific to HRNR. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of HRNR in the samples is then determined by comparing the OD of the samples to the standard curve.

技术数据

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
5000	2.458	2.489	2.474	2.451
2500	1.602	1.616	1.609	1.586
1250	0.936	0.955	0.946	0.923
625	0.417	0.425	0.421	0.398
312.5	0.208	0.21	0.209	0.186
156.25	0.11	0.111	0.111	0.088
78.13	0.058	0.058	0.058	0.035
0	0.023	0.023	0.023	0

精密度

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

回收率

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

样本类型	Range(%)	Average Recovery(%)
Serum (n=8)	84-93	87
EDTA plasma (n=8)	95-108	101
Cell culture media (n=8)	82-97	89

线性

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	80-102	88-103	95-102
1:2	Average (%)	82	90	100
1:4	Range (%)	83-96	88-103	80-91
1:4	Average (%)	89	101	80
1:8	Range (%)	80-93	86-92	87-96
1:8	Average (%)	89	89	89
1:16	Range (%)	88-97	91-99	83-101
1:16	Average (%)	97	92	93

检测流程图