EasyPro Universal Free Triiodothyronine(fT3)ELISA Kit

Universal

FT3

Competitive

60000fg/mL

258.3 fg/mL

937.5-60000 fg/mL

45min

Serum, plasma and other biological fluids

Signal transduction;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with fT3 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for fT3 are added to the micro ELISA plate wells. fT3 in samples (or standards) competes with a fixed amount of fT3 on the solid phase supporter for sites on the HRP linked detection antibody specific to fT3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.