EasyPro Rat Thiobarbituric Acid Reactive Substance(TBARS)ELISA Kit

Rat

#N/A

accfv

Competitive

400nmol/ml

3.55 nmol/ml

6.25-400nmol/ml

45min

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Metabolic pathway;Hepatology;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TBARS Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for TBARS are added to the micro ELISA plate wells. TBARS in samples (or standards) competes with a fixed amount of TBARS on the solid phase supporter for sites on the HRP linked detection antibody specific to TBARS. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.