EasyPro Universal Vitamin D2(VD2)ELISA Kit

Universal

Ergocalciferol; Deltalin; Drisdol; Calcidol

Competitive

100ng/mL

0.53 ng/mL

1.57-100 ng/mL

45min

Serum, plasma and other biological fluids.

Metabolic pathway;Neuro science;Nutrition metabolism;Bone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VD2 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for VD2 are added to the micro ELISA plate wells. VD2 in samples (or standards) competes with a fixed amount of VD2 on the solid phase supporter for sites on the HRP linked detection antibody specific to VD2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of VD2 in the samples is then determined by comparing the OD of the samples to the standard curve.