EasyPro Universal Prostaglandin E2(PGE2)ELISA Kit

Universal

PG-E2

Competitive

200pg/mL

0.97 pg/mL

3.13-200 pg/mL

45min

serum, plasma and other biological fluids

Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE2 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for PGE2 are added to the micro ELISA plate wells. PGE2 in samples (or standards) competes with a fixed amount of PGE2 on the solid phase supporter for sites on the HRP linked detection antibody specific to PGE2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve.