EasyPro Universal Malondialdehyde(MDA)ELISA Kit

Universal

MDA

Competitive

2000ng/mL

9.15 ng/mL

31.25-2000 ng/mL

45min

serum, plasma and other biological fluids

Metabolic pathway;Hepatology;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MDA Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for MDA are added to the micro ELISA plate wells. MDA in samples (or standards) competes with a fixed amount of MDA on the solid phase supporter for sites on the HRP linked detection antibody specific to MDA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of MDA in the samples is then determined by comparing the OD of the samples to the standard curve.