EasyPro Universal Androsterone(ADT)ELISA Kit

Universal

17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone

Competitive

90000pg/mL

144 pg/mL

1406.25-90000 pg/mL

45min

serum, plasma and other biological fluids

Endocrinology;Reproductive science;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ADT Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for ADT are added to the micro ELISA plate wells. ADT in samples (or standards) competes with a fixed amount of ADT on the solid phase supporter for sites on the HRP linked detection antibody specific to ADT. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of ADT in the samples is then determined by comparing the OD of the samples to the standard curve.