EasyPro Universal Acetylcholine(ACH)ELISA Kit

Universal

Acetylcholine

Competitive

1000pg/mL

5.31 pg/mL

15.63-1000 pg/mL

45min

serum, plasma and other biological fluids

Signal transduction;Infection immunity;Neuro science;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ACH Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for ACH are added to the micro ELISA plate wells. ACH in samples (or standards) competes with a fixed amount of ACH on the solid phase supporter for sites on the HRP linked detection antibody specific to ACH. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of ACH in the samples is then determined by comparing the OD of the samples to the standard curve.