EasyPro Universal Estrone Sulfate(E1S)ELISA Kit

Universal

E1S

Competitive

100ng/mL

0.51 ng/mL

1.57-100 ng/mL

45min

Serum, plasma and other biological fluids.

Reproductive science;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E1S Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for E1S are added to the micro ELISA plate wells. E1S in samples (or standards) competes with a fixed amount of E1S on the solid phase supporter for sites on the HRP linked detection antibody specific to E1S. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of E1S in the samples is then determined by comparing the OD of the samples to the standard curve.