EasyPro Universal Pentosidine(PTD)ELISA Kit

Universal

PTD

Competitive

400ng/mL

2.13 ng/mL

6.25-400 ng/mL

45min

Serum, plasma and other biological fluids

Metabolic pathway;Endocrinology;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PTD Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for PTD are added to the micro ELISA plate wells. PTD in samples (or standards) competes with a fixed amount of PTD on the solid phase supporter for sites on the HRP linked detection antibody specific to PTD. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of PTD in the samples is then determined by comparing the OD of the samples to the standard curve.